Journal: Journal of Virology

Article Title: T Cell Responses Induced by Attenuated Flavivirus Vaccination Are Specific and Show Limited Cross-Reactivity with Other Flavivirus Species

doi: 10.1128/JVI.00089-20

Figure Lengend Snippet: Antibody panel used in flow cytometry experiments to identify both magnitude and quality of CD8 + T cell response and relevant subpopulations a

Article Snippet: Detailed information on all the antibodies used for flow cytometry experiments in this study can be found in . table ft1 table-wrap mode="anchored" t5 TABLE 4 caption a7 Antibody Fluorochrome Vol (ml)/test Vendor Catalog Clone CD4 APC ef 780 1 eBioscience 47-0049-42 RPA-T4 CD3 AF700 2 Biolegend 317340 OKT3 CD8 BV650 2 BioLegend 301042 RPA-T8 CD14 V500 2 BD Biosciences 561391 M5E2 CD19 V500 2 BD Biosciences 561121 HIB19 Fixability dye ef506 1 μl/ml of master mix eBioscience 65-0866-18 NA IFN-γ FITC 1 eBioscience 11-7319-82 4S.B3 CD154 (CD40L) PE 2 eBioscience 12-1548-42 24-31 CD69 PE Cy7 2 eBioscience 25-0699-42 FN50 CD137(4-1BB) APC 2 BioLegend 309810 4B4-1 Open in a separate window a Abbreviations: APC, allophycocyanin; PE, phycoerythrin; FITC, fluorescein isothiocyanate; NA, not applicable.

Techniques: Flow Cytometry